Advances on the Transfer of Lipids by Lipid Transfer Proteins

Transfer of lipid across the cytoplasm is an essential process for intracellular lipid traffic. Lipid transfer proteins (LTPs) are defined by highly controlled in vitro experiments. The functional relevance of these is supported by evidence for the same reactions inside cells. Major advances in the LTP field have come from structural bioinformatics identifying new LTPs, and from the development of countercurrent models for LTPs. However, the ultimate aim is to unite in vitro and in vivo data, and this is where much progress remains to be made. Even where in vitro and in vivo experiments align, rates of transfer tend not to match. Here we set out some of the advances that might test how LTPs work

Peculiar behaviour of optical polarization gratings in light-sensitive liquid crystalline elastomers

The angular dependence of the diffraction efficiency of volumetype holographic gratings recorded in a single-domain light-sensitive liquid crystalline elastomer was investigated. Usually this dependence is expected to be very similar for intensity gratings and for polarization gratings. However, our measurements resolved a profound difference between the two types of the gratings: a typical Bragg peak of the diffraction efficiency is observed only for intensity gratings, while polarization gratings exhibit a profound dip at the Bragg angle. The appearance of this dip is explained by strongly anisotropic optical absorption of the actinic light during the recording process

Traffic of p24 Proteins and COPII Coat Composition Mutually Influence Membrane Scaffolding

SummaryEukaryotic protein secretion requires efficient and accurate delivery of diverse secretory and membrane proteins. This process initiates in the ER, where vesicles are sculpted by the essential COPII coat. The Sec13p subunit of the COPII coat contributes to membrane scaffolding, which enforces curvature on the nascent vesicle. A requirement for Sec13p can be bypassed when traffic of lumenally oriented membrane proteins is abrogated. Here we sought to further explore the impact of cargo proteins on vesicle formation. We show that efficient ER export of the p24 family of proteins is a major driver of the requirement for Sec13p. The scaffolding burden presented by the p24 complex is met in part by the cargo adaptor Lst1p, which binds to a subset of cargo, including the p24 proteins. We propose that the scaffolding function of Lst1p is required to generate vesicles that can accommodate difficult cargo proteins that include large oligomeric assemblies and asymmetrically distributed membrane proteins. Vesicles that contain such cargoes are also more dependent on scaffolding by Sec13p, and may serve as a model for large carrier formation in other systems

Slow stress relaxation in randomly disordered nematic elastomers and gels

Randomly disordered (polydomain) liquid crystalline elastomers align under stress. We study the dynamics of stress relaxation before, during and after the Polydomain-Monodomain transition. The results for different materials show the universal ultra-slow logarithmic behaviour, especially pronounced in the region of the transition. The data is approximated very well by an equation Sigma(t) ~ Sigma_{eq} + A/(1+ Alpha Log[t]). We propose a theoretical model based on the concept of cooperative mechanical resistance for the re-orientation of each domain, attempting to follow the soft-deformation pathway. The exact model solution can be approximated by compact analytical expressions valid at short and at long times of relaxation, with two model parameters determined from the data.Comment: 4 pages (two-column), 5 EPS figures (included via epsfig

Spontaneous Liquid Crystal and Ferromagnetic Ordering of Colloidal Magnetic Nanoplates

Ferrofluids are familiar as colloidal suspensions of ferromagnetic nanoparticles in aqueous or organic solvents. The dispersed particles are randomly oriented but their moments become aligned if a magnetic field is applied, producing a variety of exotic and useful magneto-mechanical effects. A longstanding interest and challenge has been to make such suspensions macroscopically ferromagnetic, that is having uniform magnetic alignment in absence of a field. Here we report a fluid suspension of magnetic nanoplates which spontaneously aligns into an equilibrium nematic liquid crystal phase that is also macroscopically ferromagnetic. Its zero-field magnetization produces distinctive magnetic self-interaction effects, including liquid crystal textures of fluid block domains arranged in closed flux loops, and makes this phase highly sensitive, with it dramatically changing shape even in the Earth's magnetic field

New molecular mechanisms of inter-organelle lipid transport

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Solids on Soli: Millimetre-Wave Radar Sensing through Materials

International audienceGesture recognition with miniaturised radar sensors has received increasing attention as a novel interaction medium. The practical use of radar technology, however, often requires sensing through materials. Yet, it is still not well understood how the internal structure of materials impacts recognition performance. To tackle this challenge, we collected a large dataset of 14,090 radar recordings for 6 paradigmatic gesture classes sensed through a variety of everyday materials, performed by humans (6 materials) and a robot system (75 materials). Next, we developed a hybrid CNN+LSTM deep learning model and derived a robust indirect method to measure signal distortions, which we used to compile a comprehensive catalogue of materials for radar-based interaction. Among other findings, our experiments show that it is possible to estimate how different materials would affect gesture recognition performance of arbitrary classifiers by selecting just 3 reference materials. Our catalogue, software, models, data collection platform, and labeled datasets are publicly available